How Can We Obtain Cells for Cell Culture

For T cells interleukin-2 IL-2 is a potent cytokine which modulates proliferation and differentiation into effector and memory T cells. We used three human breast cancer cell lines BT-474 MCF-7 and Hs578T as the target cells and one human NK cell line as the effector cells.

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The type of culture media for successful cultivation can vary depending on the type of cell or subset of cell you require.

. We first measured the levels of HER2neu expression on. Culture conditions may be further refined to polarize T cells to a specific phenotype during expansion.

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